转录因子MYB相关蛋白B即用型免疫组化试剂盒-分析方法-资讯-生物在线

转录因子MYB相关蛋白B即用型免疫组化试剂盒

英文名称

MYBL2 Ready-To-Use IHC Kit

中文名称

转录因子MYB相关蛋白B即用型免疫组化试剂盒

别名

B-Myb; BMyB; MYB L2; Myb related protein B; Myb-like protein 2; Myb-related protein B; MybB; MYBB_HUMAN; MYBL 2; Mybl2; v myb avian myeloblastosis viral oncogene homolog like 2; v myb myeloblastosis viral oncogene homolog (avian) like 2; v myb myeloblastosis viral oncogene homolog like 2.

种属

Human, Mouse, Rat

应用范围

Immunohistochemistry

适用样品基质

PFPE tissue

描述

IHC0105 is a ready-to-use IHC kit for staining of MYBL2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

保存条件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事项

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍

产品组分及规格

编号	组分	规格	浓度	储存
1	PBS缓冲液（干粉）	2 L×2	20x	室温
2	抗原修复缓冲液	20 ml	100x	2-8℃
3	内源性过氧化物酶阻断剂	3 ml	RTU	2-8℃
4	封闭工作液	3 ml	RTU	2-8℃
5	一抗（MYBL2 Rabbit pAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Rabbit IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）显色液	0.3 ml	RTU	-20℃
8	DAB kit（20×）稀释液	0.3 ml	RTU	-20℃
9	复染试剂	5 ml	RTU	室温
10	封片剂	5 ml	RTU	室温
11	对照切片（大鼠脑，小鼠脑，人胃癌）	3张	RTU	室温
12	说明书	1份

背景

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次，每次15 min；依次置于不同浓度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸馏水洗涤5 min，重复3次。

2. 抗原修复

沸水浴修复：将100×抗原修复缓冲液（试剂2）用蒸馏水稀释成1×抗原修复缓冲液，放入修复盒中并提前加热至95-100℃（注意盖好以防液体蒸发），然后将切片放入修复盒中，在沸水浴环境中保持外沸状态15 min，室温自然冷却；用PBS缓冲液（试剂1，将干粉溶解在2L蒸馏水中）清洗5 min，重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体，用免疫组化笔在组织周围画圈，加入2-4滴内源性过氧化物酶阻断剂（试剂3），室温下置于湿盒中孵育15 min，用PBS洗涤5 min，重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体，加入2-4滴封闭工作液（试剂4），置于湿盒内37℃封闭20 min，以减少非特异性染色。

5. 一抗孵育

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