PDHX (C-term) Peptide
产品名称： PDHX (C-term) Peptide
英文名称： PDHX (C-term) Peptide
产品编号： P0826
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PDHX.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PDHX.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Quantitative proteomics: the copy number of pyruvate dehydrogenase is more than 10(2)-fold lower than that of complex III in human mitochondria.
Murray J, Gilkerson R, Capaldi RA.FEBS Lett. 2002 Oct 9;529(2-3):173-8.
- 2.
- Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency.
Ling M, McEachern G, Seyda A, MacKay N, Scherer SW, Bratinova S, Beatty B, Giovannucci-Uzielli ML, Robinson BH.Hum Mol Genet. 1998 Mar;7(3):501-5.
- 3.
- Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex.
Harris RA, Bowker-Kinley MM, Wu P, Jeng J, Popov KM.J Biol Chem. 1997 Aug 8;272(32):19746-51.
- Applications
- Western Blot (Tissue lysate)
- The PDHX polyclonal antibody (Cat # PAB3860) is used in Western blot to detect PDHX in mouse heart tissue lysate.
- Western Blot (Transfected lysate)
- Western blot analysis of PDX1 (arrow) using rabbit PDHX polyclonal antibody (Cat # PAB3860). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDHX polyclonal antibody (Cat # PAB3860) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Entrez GeneID:
- 8050
- Protein Accession#:
- NP_003468;O00330
- Gene Name:
- PDHX
- Gene Alias:
- DLDBP,E3BP,OPDX,PDX1,proX
- Gene Description:
- pyruvate dehydrogenase complex, component X
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
- Other Designations:
- E3-binding protein,pyruvate dehydrogenase complex, lipoyl-containing component X