PAK6 polyclonal antibody
产品名称： PAK6 polyclonal antibody
英文名称： PAK6 polyclonal antibody
产品编号： PAB2303
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PAK6.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 131-146 of human PAK6.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Identification of an autoinhibitory domain of p21-activated protein kinase 5.
Ching YP, Leong VY, Wong CM, Kung HF.J Biol Chem. 2003 Sep 5;278(36):33621-4. Epub 2003 Jul 14.
- 2.
- Cloning and characterization of PAK5, a novel member of mammalian p21-activated kinase-II subfamily that is predominantly expressed in brain.
Pandey A, Dan I, Kristiansen TZ, Watanabe NM, Voldby J, Kajikawa E, Khosravi-Far R, Blagoev B, Mann M.Oncogene. 2002 May 30;21(24):3939-48.
- 3.
- Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6.
Yang F, Li X, Sharma M, Zarnegar M, Lim B, Sun Z.J Biol Chem. 2001 May 4;276(18):15345-53. Epub 2001 Jan 25.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of PAK6 polyclonal antibody (Cat # PAB2303) in lysates from transiently transfected COS-7 cells.
Lane 1 : negative control.
Lane 2 : PAK1-expressing cells.
Lane 3 : PAK2-expressing cells
Lane 4 : PAK4-expressing cells.
Lane 5 : PAK5-expressing cells.
Lane 6 : PAK6-expressing cells.
PAK6 (arrow) was detected using purified polyclonal antibody. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA) .
- Entrez GeneID:
- 56924
- Protein Accession#:
- NP_064553
- Gene Name:
- PAK6
- Gene Alias:
- PAK5
- Gene Description:
- p21 protein (Cdc42/Rac)-activated kinase 6
- Omim ID:
- 608110
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
- Other Designations:
- p21(CDKN1A)-activated kinase 6,p21-activated kinase 6,p21-activated protein kinase 6