糜蛋白酶原B2(CTRB2)重组蛋白

YB214Hu013
Recombinant Chymotrypsinogen B2 (CTRB2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
糜蛋白酶原B2(CTRB2)重组蛋白[ PROPERTIES ]
Residues: Ile34~Asn263
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q6GPI1
Host: E. coli
Subcellular Location: Secreted, extracellular
space.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
糜蛋白酶原B2(CTRB2)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.7
Predicted Molecular Mass: 54.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
糜蛋白酶原B2(CTRB2)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
IVNGEDA VPGSWPWQVS LQDKTGFHFC GGSLISEDWV VTAAHCGVRT SDVVVAGEFD
QGSDEENIQV LKIAKVFKNP KFSILTVNND ITLLKLATPA RFSQTVSAVC LPSADDDFPA
GTLCATTGWG KTKYNANKTP DKLQQAALPL LSNAECKKSW GRRITDVMIC AGASGVSSCM
GDSGGPLVCQ KDGAWTLVGI VSWGSRTCST TTPAVYARVA KLIPWVQKIL AAN