ZNF274 monoclonal antibody (M04), clone 1D8
产品名称： ZNF274 monoclonal antibody (M04), clone 1D8
英文名称： ZNF274 monoclonal antibody (M04), clone 1D8
产品编号： H00010782-M04
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant ZNF274.
- Immunogen:
- ZNF274 (NP_598009, 420 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- QKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHT
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M04), clone 1D8.
Lane 1: ZNF274 transfected lysate(74.2 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to ZNF274 on HeLa cell . [antibody concentration 10 ug/ml]
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of ZNF274 over-expressed 293 cell line, cotransfected with ZNF274 Validated Chimera RNAi ( Cat # H00010782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF274 monoclonal antibody (M04), clone 1D8 (Cat # H00010782-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 10782
- GeneBank Accession#:
- NM_133502
- Protein Accession#:
- NP_598009
- Gene Name:
- ZNF274
- Gene Alias:
- DKFZp686K08243,FLJ37843,HFB101,ZF2,ZKSCAN19
- Gene Description:
- zinc finger protein 274
- Omim ID:
- 605467
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq
- Other Designations:
- KRAB zinc finger protein HFB101,zinc finger protein zfp2
- Gene Pathway