Description:
Bacteria protein extraction kit is designed for the lysis of bacterial cells for the purification of recombinant and wild type proteins. It is useful for the preparation of high purity inclusion bodies. There is no need for special equipment such as sonicator. This method is fast and obtains high yields of protein. The gentle, non-ionic extraction method can be used to extract functionally soluble proteins without a need for secondary treatment such as sonication or freeze/thaw. It is compatible with the FLAG, poly-His and GST fusion protein affinity chromatography purification systems, the kit is also compatible with many enzyme or protein assays and compatible with protease inhibitor cocktails.
Applications: The kit provides reagent for extracting bacterial protein from 1-5 liter culture and 10 or 50 extractions.
Features: Simple & Fast, noi need for special equipment.It is suitable for long term storage.
Storage Condition: Store 10x Cell lysis buffer at 4℃;DNase I, RNase, Lysozyme and PMSF should be stored at -20℃.