研究领域 肿瘤  信号转导  通道蛋白  新陈代谢   

抗体来源 Rabbit 

克隆类型 Polyclonal 

交叉反应 Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, ) 

产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 28kDa 

细胞定位 细胞膜  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human AQP1:181-269/269  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Function:

Forms a water-specific channel that provides the plasmamembranes of red cells and kidney proximal tubules with highpermeability to water, thereby permitting water to move in thedirection of an osmotic gradient.

Subunit:

Homotetramer. Interacts with EPHB2; involved in endolymphproduction in the inner ear (By similarity).

Subcellular Location:

Membrane; Multi-pass membrane protein.

Tissue Specificity:

Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.

Similarity:

Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:

P29972

Gene ID:

358

Database links:

Entrez Gene: 358 HumanEntrez Gene: 11826 MouseEntrez Gene: 25240 RatEntrez Gene: 442999 SheepEntrez Gene: 282653 CowEntrez Gene: 403732 DogOmim: 107776 HumanSwissProt: P47865 CowSwissProt: 

产品图片

Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-AQP1 (bs-1506R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP-1 Polyclonal Antibody, Unconjugated(bs-1506R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: 293T (blue). Primary Antibody:Rabbit Anti-AQP1 antibody(bs-1506R), Dilution: 1μg in 100μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1506R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.