GGT1 monoclonal antibody (M01), clone 1F9
产品名称： GGT1 monoclonal antibody (M01), clone 1F9
英文名称： GGT1 monoclonal antibody (M01), clone 1F9
产品编号： H00002678-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant GGT1.
- Immunogen:
- GGT1 (NP_005256, 381 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- TAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVG
- Host:
- Mouse
- Reactivity:
- Human, Mouse
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Autocatalytic cleavage of human {gamma}-glutamyl transpeptidase is highly dependent on N-glycosylation at asparagine 95.
West MB, Wickham S, Quinalty LM, Pavlovicz RE, Li C, Hanigan MH.J Biol Chem. 2011 Jun 28. [Epub ahead of print]
- 2.
- Analysis of site-specific glycosylation of renal and hepatic ?^-glutamyl transpeptidase from normal human tissue.
West MB, Segu ZM, Feasley CL, Kang P, Klouckova I, Li C, Novotny MV, West CM, Mechref Y, Hanigan MH.J Biol Chem. 2010 Sep 17;285(38):29511-24. Epub 2010 Jul 9.
- Applications
- Western Blot (Cell lysate)
- GGT1 monoclonal antibody (M01), clone 1F9 Western Blot analysis of GGT1 expression in NIH/3T3 ( Cat # L018V1 ).
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to GGT1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of GGT1 transfected lysate using anti-GGT1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GGT1 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged GGT1 is 0.1 ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 2678
- GeneBank Accession#:
- NM_005265
- Protein Accession#:
- NP_005256
- Gene Name:
- GGT1
- Gene Alias:
- CD224,D22S672,D22S732,GGT,GTG,MGC96892,MGC96904,MGC96963
- Gene Description:
- gamma-glutamyltransferase 1
- Omim ID:
- 231950
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
- Other Designations:
- OTTHUMP00000028921,OTTHUMP00000159078,gamma-glutamyl transpeptidase,glutamyl transpeptidase