IdeS Protease (IgG specific)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Concentration

20000U/mg

Purity

90% as determined by SDS-PAGE

Applications

Proteomics

Endotoxin level

Please contact with the lab for this information.

Expression system

E. coli

Protein length

IdeS Protease is cloned from Streptococcus pyogenes and expressed inE.coli.

Nature

Recombinant

Predicted molecular weight

37.57 kDa

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Storeat 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months fromthe date of receipt.

Species

Streptococcus pyogenes

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid.

They are shipped out with dry ice/blue ice unless customers requireotherwise.

Note

For research use only.

Description

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It cleavesImmunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 andFc fragments.

Precision

Unit Definition

One unit will cleave ≥95% of 1μg of recombinant monoclonal IgG in 30 minutes at 37°C.

Digestion reaction condition37℃, 30min

Protocol

1. Add appropriate amount of IgG (to 5mg) in digestive juice;

2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG;

3. Incubate the sample at 37°C for 30-60min.

IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, suchas Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but theincubation time or enzyme amount needs to be optimized according to the actualsituation.

Data Image

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